Computational Biology and Bioinformatics

Volume 1, Issue 6, December 2013

  • Efficacy of Bisulfite Modification and DNA Recovery Using Commercial Kits from Samples of Genomic and Circulating DNA

    Olga Bryzgunova, Pavel Laktionov, Тatyana Skvortsova, Anna Bondar, Evgeniy Morozkin, Alena Lebedeva, Valentin Vlassov, Kurt Miller, Hans Krause

    Issue: Volume 1, Issue 6, December 2013
    Pages: 28-36
    Received: 24 February 2013
    Published: 20 December 2013
    Abstract: This study had evaluated three commercially available bisulfite modification kits with regard to the procedural DNA loss and the efficacy of cytosine conversion to uracil. The efficacy of DNA conversion was estimated by pyrosequencing of eight CpG sites of unmethylated and fully enzymatically methylated templates of RARβ gene promoter region and ci... Show More